PIEZO1 Knockout cell line(BEAS-2B)

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PIEZO1 Knockout cell line(BEAS-2B) Order

PIEZO1 Knockout cell line(BEAS-2B)
Catalog#
YKO-H054
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIEZO1 Knockout cell line(BEAS-2B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PIEZO1 Knockout cell line (BEAS-2B)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
PIEZO1
Gene ID
9780
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 20,Exon 21,Exon 22,Exon 23 (based on transcript PIEZO1-201)
Validation: PCR+Sanger Sequencing
PIEZO1 Knockout cell line(BEAS-2B) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:BEAS-2B
STR Info (Cell bank)
Cell Line:BEAS-2B
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 13131313
D7S820 10131013
D16S539 12121212
VWA 17181718
TH01 79.379.3
AMEL XYXY
TPOX 611611
CSF1PO 912912
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIEZO1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BEAS-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIEZO1 gene knockout PIEZO1 Knockout cell line (BEAS-2B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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