PIGA Knockout cell line (K-562)

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PIGA Knockout cell line (K-562) Order

PIGA Knockout cell line (K-562)
Catalog#
YKO-H109
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIGA Knockout cell line (K-562)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PIGA Knockout cell line (K-562)
Morphology
Lymphocyte-like,suspension
Passage ratio
1:3-1:5
Species
Human
Gene Name
PIGA
Gene ID
5277
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%IMDM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript PIGA-201)
Validation: PCR+Sanger Sequencing
PIGA Knockout cell line (K-562) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:K-562
STR Info (Cell bank)
Cell Line:K-562
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 8888
D7S820 911911
D16S539 11121112
VWA 16161616
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8989
CSF1PO 910910
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIGA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the K-562 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIGA gene knockout PIGA Knockout cell line (K-562) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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