PKD2 Knockout cell line(H1)

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PKD2 Knockout cell line(H1) Order

PKD2 Knockout cell line(H1)
Catalog#
YKO-H319
Size
2*10^5
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PKD2 Knockout cell line(H1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PKD2 Knockout cell line (H1)
Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
PKD2
Gene ID
5311
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Target region
Knockout region: Exon 3 (based on transcript PKD2-201)
Validation: PCR+Sanger Sequencing
PKD2 Knockout cell line(H1) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:H1		 STR Info (Cell bank)
Cell Line:WAe001-A-16
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 911911
D13S317 811811
D7S820 812812
D16S539 913913
VWA 15171517
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PKD2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PKD2 gene knockout PKD2 Knockout cell line (H1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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