PKM Knockout cell line(HeLa)

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location： Home > Products > KO Cell Lines > PKM Knockout cell line(HeLa)

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PKM Knockout cell line(HeLa) Order

PKM Knockout cell line(HeLa)
Catalog#
YKO-H033
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PKM Knockout cell line(HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PKM Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
PKM
Gene ID
5315
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Accession
CVCL_E0KZ(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 2 (based on transcript PKM-202)
Validation: PCR+Sanger Sequencing
PKM Knockout cell line(HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PKM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PKM gene knockout PKM Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PKM Knockout cell line(HeLa)
PKM Knockout cell line(HeLa)
Catalog#
YKO-H033
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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