PKM Knockout cell line(MDA-MB-231)

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PKM Knockout cell line(MDA-MB-231) Order

PKM Knockout cell line(MDA-MB-231)
Catalog#
YKO-H014
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PKM Knockout cell line(MDA-MB-231)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PKM Knockout cell line (MDA-MB-231)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PKM
Gene ID
5315
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%L-15+10%FBS(Recommend to use Ubigene-optimized culture medium)
Target region
Knockout region: Exon 2 (based on transcript PKM-202)
Validation: PCR+Sanger Sequencing
PKM Knockout cell line(MDA-MB-231) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:MDA-MB-231
STR Info (Cell bank)
Cell Line:MDA-MB-231
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 13131313
D7S820 8989
D16S539 12121212
VWA 15181518
TH01 79.379.3
AMEL XXXX
TPOX 8989
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PKM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDA-MB-231 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PKM gene knockout PKM Knockout cell line (MDA-MB-231) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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