PRNP Knockout cell line(A549)

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PRNP Knockout cell line(A549) Order

PRNP Knockout cell line(A549)
Catalog#
YKO-H088
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PRNP Knockout cell line(A549)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PRNP Knockout cell line (A549)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
PRNP
Gene ID
5621
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% F12K+10% FBS
Target region
Knockout region: Exon 2 (based on transcript PRNP-201)
Validation: PCR+Sanger Sequencing
PRNP Knockout cell line(A549) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:A549
STR Info (Cell bank)
Cell Line:A-549
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 811811
D16S539 11121112
VWA 14141414
TH01 89.389.3
AMEL XYXY
TPOX 811811
CSF1PO 10121012
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PRNP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A549 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PRNP gene knockout PRNP Knockout cell line (A549) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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