PTPRF Knockout cell line(U251)

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PTPRF Knockout cell line(U251) Order

PTPRF Knockout cell line(U251)
Catalog#
YKO-H194
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PTPRF Knockout cell line(U251)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PTPRF Knockout cell line (U251)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
PTPRF
Gene ID
5792
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 9 (based on transcript PTPRF-201)
Validation: PCR+Sanger Sequencing
PTPRF Knockout cell line(U251) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U251
STR Info (Cell bank)
Cell Line:U251-MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10111011
D7S820 10121012
D16S539 12121212
VWA 16181618
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PTPRF gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U251 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PTPRF gene knockout PTPRF Knockout cell line (U251) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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