SENP7 Knockout cell line(SK-BR-3)

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SENP7 Knockout cell line(SK-BR-3) Order

SENP7 Knockout cell line(SK-BR-3)
Catalog#
YKO-HJ064
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SENP7 Knockout cell line(SK-BR-3)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SENP7 Knockout cell line (SK-BR-3)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:3
Species
Human
Gene Name
SENP7
Gene ID
57337
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10%FBS
Accession
CVCL_E1C7(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 4 (based on transcript SENP7-207)
Validation: PCR+Sanger Sequencing
SENP7 Knockout cell line(SK-BR-3) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:SK-BR-3
STR Info (Cell bank)
Cell Line:SK-BR-3
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 912912
D13S317 11121112
D7S820 99912
D16S539 9999
VWA 17171717
TH01 8989
AMEL XXXX
TPOX 811811
CSF1PO 12121212
D12S391 1820
FGA 2020
D2S1338 2025
D21S11 3030.2
D18S51 1013
D8S1179 1212
D3S1358 1717
D6S1043 1119
PENTAE 1011
D19S433 1414
PENTAD 912
D1S1656 1117.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SENP7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SK-BR-3 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SENP7 gene knockout SENP7 Knockout cell line (SK-BR-3) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SENP7 Knockout cell line(SK-BR-3)
SENP7 Knockout cell line(SK-BR-3)
Catalog#
YKO-HJ064
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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