SETD2 Knockout cell line(CNE1)

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SETD2 Knockout cell line(CNE1) (Hela-contaminated cells, not for sales) Order

SETD2 Knockout cell line(CNE1)
Catalog#
YKO-H044
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SETD2 Knockout cell line(CNE1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SETD2 Knockout cell line (CNE1)
Morphology
Endothelial cells, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
SETD2
Gene ID
29072
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:CNE1
STR Info (Cell bank)
Cell Line:CNE-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 101213.3101213.3
D7S820 10121012
D16S539 910910
VWA 14161416
TH01 679.0679.0
AMEL XXXX
TPOX 812812
CSF1PO 10111011
* Hela-contaminated cells, not for sales
Reviewed by ICLAC (ref: 121113, 150506). STR profiling in Chan et al (2008) demonstrated similarity to HeLa, but at less than 80% match. Strong et al (2014) presented additional data in support of this finding, demonstrating that cells carry HPV-18, as previously demonstrated in HeLa cells. The authors speculate that a fusion event may have occurred between HeLa and an unknown cell line; although not proven, current data are consistent with this hypothesis.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SETD2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the CNE1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SETD2 gene knockout SETD2 Knockout cell line (CNE1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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