SGK1 Knockout cell line(SU-DHL-4)

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SGK1 Knockout cell line(SU-DHL-4) Order

SGK1 Knockout cell line(SU-DHL-4)
Catalog#
YKO-H188
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SGK1 Knockout cell line(SU-DHL-4)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SGK1 Knockout cell line (SU-DHL-4)
Morphology
Lymphoblast,suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Gene Name
SGK1
Gene ID
6446
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Target region
Knockout region: Exon 4,Exon 5,Exon 6,Exon 7,Exon 8,Exon 9,Exon 10,Exon 11,Exon 12,Exon 13,Exon 14 (based on transcript SGK1-204)
Validation: PCR+Sanger Sequencing
SGK1 Knockout cell line(SU-DHL-4) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SU-DHL-4
STR Info (Cell bank)
Cell Line:SU-DHL-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 811811
D16S539 11131113
VWA 18191819
TH01 69.369.3
AMEL XYXY
TPOX 911911
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SGK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SU-DHL-4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SGK1 gene knockout SGK1 Knockout cell line (SU-DHL-4) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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