SLC25A5 Knockout cell line(KYSE-150)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > SLC25A5 Knockout cell line(KYSE-150)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

SLC25A5 Knockout cell line(KYSE-150) Order

SLC25A5 Knockout cell line(KYSE-150)
Catalog#
YKO-H138
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SLC25A5 Knockout cell line(KYSE-150)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SLC25A5 Knockout cell line (KYSE-150)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SLC25A5
Gene ID
292
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript SLC25A5-201)
Validation: PCR+Sanger Sequencing
SLC25A5 Knockout cell line(KYSE-150) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-150
STR Info (Cell bank)
Cell Line:KYSE-150
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 811811
D7S820 10111011
D16S539 911911
VWA 16171617
TH01 7979
AMEL XXXX
TPOX 8888
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC25A5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-150 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC25A5 gene knockout SLC25A5 Knockout cell line (KYSE-150) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

KO Cell Lines

  • SLC25A5 Knockout cell line (Hela)

    SLC25A5 Knockout cell line (Hela)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS7737
    YKO-HS7737(Hela)
  • SLC25A5 Knockout cell line (HCT 116)

    SLC25A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS6853
    YKO-HS6853(HCT 116)
  • SLC25A5 Knockout cell line (HEK293)

    SLC25A5 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS5969
    YKO-HS5969(HEK293)

Related Popular Products

Wild-type Cell Lines

  • KYSE-150 cell line

    Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)

    Price (USD): -
    Catalog#: YC-B002
    Size: 1*10^6
    Instruction: Human Esophageal Squamous Carcinoma Cell Line(KYSE-150)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • SLC25A5 Knockout cell line (Hela)

    SLC25A5 Knockout cell line (Hela)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS7737
  • SLC25A5 Knockout cell line (HCT 116)

    SLC25A5 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS6853
  • SLC25A5 Knockout cell line (HEK293)

    SLC25A5 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: SLC25A5
    Gene ID: 292
    Size: 1*10^6
    Catalog#: YKO-HS5969

Contact us