SLC25A5 Knockout cell line(KYSE-150)

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SLC25A5 Knockout cell line(KYSE-150) Order

SLC25A5 Knockout cell line(KYSE-150)
Catalog#
YKO-H138
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SLC25A5 Knockout cell line(KYSE-150)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SLC25A5 Knockout cell line (KYSE-150)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SLC25A5
Gene ID
292
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript SLC25A5-201)
Validation: PCR+Sanger Sequencing
SLC25A5 Knockout cell line(KYSE-150) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-150
STR Info (Cell bank)
Cell Line:KYSE-150
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 811811
D7S820 10111011
D16S539 911911
VWA 16171617
TH01 7979
AMEL XXXX
TPOX 8888
CSF1PO 12131213
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC25A5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-150 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC25A5 gene knockout SLC25A5 Knockout cell line (KYSE-150) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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