SMAD4 Knockout cell line(HTR-8/SVneo)

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SMAD4 Knockout cell line(HTR-8/SVneo) Order

SMAD4 Knockout cell line(HTR-8/SVneo)
Catalog#
YKO-H087
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SMAD4 Knockout cell line(HTR-8/SVneo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SMAD4 Knockout cell line (HTR-8/SVneo)
Morphology
Endothelial cells, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SMAD4
Gene ID
4089
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript SMAD4-201)
Validation: PCR+Sanger Sequencing
SMAD4 Knockout cell line(HTR-8/SVneo) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HTR-8/SVneo
STR Info (Cell bank)
Cell Line:HTR-8/SVneo
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 912912
D7S820 12121212
D16S539 11131313
VWA 13181318
TH01 69.369.3
AMEL XXXX
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SMAD4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HTR-8/SVneo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SMAD4 gene knockout SMAD4 Knockout cell line (HTR-8/SVneo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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