SNORA59B Knockout cell line(SK-MES-1)

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SNORA59B Knockout cell line(SK-MES-1) Order

SNORA59B Knockout cell line(SK-MES-1)
Catalog#
YKO-H165
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SNORA59B Knockout cell line(SK-MES-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SNORA59B Knockout cell line (SK-MES-1)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
SNORA59B
Gene ID
677882
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%MEM+10% FBS
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:SK-MES-1
STR Info (Cell bank)
Cell Line:SK-MES-1
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11111111
D7S820 8888
D16S539 13131313
VWA 14141414
TH01 69.369.3
AMEL XYXY
TPOX 8888
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SNORA59B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the SK-MES-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SNORA59B gene knockout SNORA59B Knockout cell line (SK-MES-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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