STX17 Knockout cell line(266-6)

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STX17 Knockout cell line(266-6) Order

STX17 Knockout cell line(266-6)
Catalog#
YKO-H199
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
STX17 Knockout cell line(266-6)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
STX17 Knockout cell line (266-6)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
STX17
Gene ID
55014
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:266-6
STR Info (Cell bank)
Cell Line:266-6
Allele1Allele2Allele3Allele1Allele2Allele3
4-2 233.47
【19.3】
237.47
【20.3】
233.88
【19.3】
237.98
【20.3】
5-5 335.49
【14】
335.95
【14】
6-4 299.77
【18】
300.24
【18】
6-7 334.09
【12】
354.34
【17】
334.49
【12】
354.61
【17】
9-2 233.19
【18】
233.62
【18】
12-1 229.59
【17】
230.15
【17】
15-3 200.72
【22.3】
201
【22.3】
18-3 151.65
【16】
152.12
【16】
X-1 412.26
【28】
412.79
【28】
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the STX17 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 266-6 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, STX17 gene knockout STX17 Knockout cell line (266-6) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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