TLR3 Knockout cell line(A-431)

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TLR3 Knockout cell line(A-431) Order

TLR3 Knockout cell line(A-431)
Catalog#
YKO-H072
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TLR3 Knockout cell line(A-431)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TLR3 Knockout cell line (A-431)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:5
Species
Human
Gene Name
TLR3
Gene ID
7098
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
88% DMEM+10% FBS+1%Glutamax+1%Sodium Pyruvate
Target region
Knockout region: Exon 2 (based on transcript TLR3-201)
Validation: PCR+Sanger Sequencing
TLR3 Knockout cell line(A-431) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:A-431
STR Info (Cell bank)
Cell Line:A-431
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12131213
D13S317 913913
D7S820 10101010
D16S539 12141214
VWA 15171517
TH01 9999
AMEL XXXX
TPOX 11111111
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLR3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A-431 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLR3 gene knockout TLR3 Knockout cell line (A-431) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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