TRPV6 Knockout cell line(BeWo)

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TRPV6 Knockout cell line(BeWo) Order

TRPV6 Knockout cell line(BeWo)
Catalog#
YKO-H124
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRPV6 Knockout cell line(BeWo)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRPV6 Knockout cell line (BeWo)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
TRPV6
Gene ID
55503
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
50%DMEM/F12+40%FBS+10%DMSO
Target region
Knockout region: Exon 1 (based on transcript TRPV6-201)
Validation: PCR+Sanger Sequencing
TRPV6 Knockout cell line(BeWo) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:BeWo
STR Info (Cell bank)
Cell Line:BEWO
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 911911
D7S820 10121012
D16S539 13141314
VWA 16161616
TH01 99.399.3
AMEL XYXY
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRPV6 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BeWo cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRPV6 gene knockout TRPV6 Knockout cell line (BeWo) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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