TSC2 Knockout cell line(U-87 MG)

location: Home > Products > KO Cell Lines > TSC2 Knockout cell line(U-87 MG)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

TSC2 Knockout cell line(U-87 MG) Order

TSC2 Knockout cell line(U-87 MG)
Catalog#
YKO-H205
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TSC2 Knockout cell line(U-87 MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TSC2 Knockout cell line (U-87 MG)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
TSC2
Gene ID
7249
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 5 (based on transcript TSC2-201)
Validation: PCR+Sanger Sequencing
TSC2 Knockout cell line(U-87 MG) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U-87 MG
STR Info (Cell bank)
Cell Line:U-87MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 811811
D7S820 8989
D16S539 12121212
VWA 15171517
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TSC2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U-87 MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TSC2 gene knockout TSC2 Knockout cell line (U-87 MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • U-87 MG cell line

    Human Lioblastoma Multiforme(U-87 MG)

    Catalog#: YC-C028
    Size: 1*10^6
    Instruction: Human  Lioblastoma Multiforme(U-87 MG)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TSC2 Knockout cell line (Hela)

    TSC2 Knockout cell line (Hela)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS4091
    YKO-HS4091(Hela)
  • TSC2 Knockout cell line (HEK293)

    TSC2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS1477
    YKO-HS1477(HEK293)
  • TSC2 Knockout cell line (A549)

    TSC2 Knockout cell line (A549)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS720
    YKO-HS720(A549)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TSC2 Knockout cell line (Hela)

    TSC2 Knockout cell line (Hela)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS4091
  • TSC2 Knockout cell line (HEK293)

    TSC2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS1477
  • TSC2 Knockout cell line (A549)

    TSC2 Knockout cell line (A549)

    Organism: Human
    Gene Name: TSC2
    Gene ID: 7249
    Size: 1*10^6
    Catalog#: YKO-HS720

Contact us