USP7 Knockout cell line(A-375)

location: Home > Products > KO Cell Lines > USP7 Knockout cell line(A-375)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

USP7 Knockout cell line(A-375) Order

USP7 Knockout cell line(A-375)
Catalog#
YKO-H049
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
USP7 Knockout cell line(A-375)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
USP7 Knockout cell line (A-375)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Human
Gene Name
USP7
Gene ID
7874
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 4 (based on transcript USP7-201)
Validation: PCR+Sanger Sequencing
USP7 Knockout cell line(A-375) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:A-375
STR Info (Cell bank)
Cell Line:A-375 [A375]
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 9999
D16S539 9999
VWA 16171617
TH01 8888
AMEL XXXX
TPOX 810810
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the USP7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the A-375 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, USP7 gene knockout USP7 Knockout cell line (A-375) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • A-375 cell line

    Human Malignant Melanoma Cell Line(A-375)

    Catalog#: YC-C036
    Size: 1*10^6
    Instruction: Human Malignant Melanoma Cell Line(A-375)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Usp7 Knockout cell line(BV2)

    Usp7 Knockout cell line(BV2)

    Organism: Mouse
    Gene Name: Usp7
    Gene ID: 252870
    Size: 1*10^6
    Catalog#: YKO-M155
    YKO-M155(BV2)
  • USP7 Knockout cell line (Hela)

    USP7 Knockout cell line (Hela)

    Organism: Human
    Gene Name: USP7
    Gene ID: 7874
    Size: 1*10^6
    Catalog#: YKO-HS7599
    YKO-HS7599(Hela)
  • USP7 Knockout cell line (HCT 116)

    USP7 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: USP7
    Gene ID: 7874
    Size: 1*10^6
    Catalog#: YKO-HS6715
    YKO-HS6715(HCT 116)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • Usp7 Knockout cell line(BV2)

    Usp7 Knockout cell line(BV2)

    Organism: Mouse
    Gene Name: Usp7
    Gene ID: 252870
    Size: 1*10^6
    Catalog#: YKO-M155
  • USP7 Knockout cell line (Hela)

    USP7 Knockout cell line (Hela)

    Organism: Human
    Gene Name: USP7
    Gene ID: 7874
    Size: 1*10^6
    Catalog#: YKO-HS7599
  • USP7 Knockout cell line (HCT 116)

    USP7 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: USP7
    Gene ID: 7874
    Size: 1*10^6
    Catalog#: YKO-HS6715

Contact us