WWOX Knockout cell line (iPSC)

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WWOX Knockout cell line (iPSC) Order

WWOX Knockout cell line (iPSC)
Catalog#
YKO-H224
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
WWOX Knockout cell line (iPSC)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
WWOX knockout cell line (iPSC)
Morphology
Spherical clone
Passage ratio
1:6-1:8
Species
Human
Gene Name
WWOX
Gene ID
51741
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
Target region
Knockout region: Exon 4 (based on transcript WWOX-214)
Validation: PCR+Sanger Sequencing
WWOX Knockout cell line (iPSC) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:iPSC
STR Info (Cell bank)
Cell Line:ATCC- DYR0100 hiPSC
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 11131113
D7S820 910910
D16S539 12121212
VWA 14191419
TH01 9.39.39.39.3
AMEL XYXY
TPOX 811811
CSF1PO 711711
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the WWOX gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the iPSC cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, WWOX gene knockout WWOX knockout cell line (iPSC) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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