ZNF750 Knockout cell line(KYSE-30)

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ZNF750 Knockout cell line(KYSE-30) Order

ZNF750 Knockout cell line(KYSE-30)
Catalog#
YKO-H028
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
ZNF750 Knockout cell line(KYSE-30)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
ZNF750 Knockout cell line (KYSE-30)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
ZNF750
Gene ID
79755
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 2 (based on transcript ZNF750-201)
Validation: PCR+Sanger Sequencing
ZNF750 Knockout cell line(KYSE-30) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-30
STR Info (Cell bank)
Cell Line:KYSE-30
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 9999
D7S820 11121112
D16S539 10121012
VWA 1619161819.0
TH01 9999
AMEL XXXX
TPOX 9999
CSF1PO 10101010
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the ZNF750 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-30 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, ZNF750 gene knockout ZNF750 Knockout cell line (KYSE-30) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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