L1CAM Knockout cell line(MDCK)

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L1CAM Knockout cell line(MDCK) Order

L1CAM Knockout cell line(MDCK)
Catalog#
YKO-H481
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
L1CAM Knockout cell line(MDCK)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
L1CAM Knockout cell line (MDCK)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:4
Species
Dog
Gene Name
L1CAM
Gene ID
492244
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%MEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript L1CAM-201)
Validation: PCR+Sanger Sequencing
L1CAM Knockout cell line(MDCK) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the L1CAM gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MDCK cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, L1CAM gene knockout L1CAM Knockout cell line (MDCK) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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