MAP3K1 Knockout cell line (HeLa)

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MAP3K1 Knockout cell line (HeLa) Order

MAP3K1 Knockout cell line (HeLa)
Catalog#
YKO-H454
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MAP3K1 Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MAP3K1 Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
MAP3K1
Gene ID
4214
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 3 (based on transcript MAP3K1-201)
Validation: PCR+Sanger Sequencing
MAP3K1 Knockout cell line (HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MAP3K1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MAP3K1 gene knockout MAP3K1 Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MAP3K1 Knockout cell line (HeLa)
MAP3K1 Knockout cell line (HeLa)
Catalog#
YKO-H454
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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