MRPL40 Knockout cell line (C4-2B)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > MRPL40 Knockout cell line (C4-2B)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

MRPL40 Knockout cell line (C4-2B) Order

MRPL40 Knockout cell line (C4-2B)
Catalog#
YKO-H1106
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MRPL40 Knockout cell line (C4-2B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MRPL40 Knockout cell line (C4-2B)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
MRPL40
Gene ID
64976
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript MRPL40-201)
Validation: PCR+Sanger Sequencing
MRPL40 Knockout cell line (C4-2B) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MRPL40 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the C4-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MRPL40 gene knockout MRPL40 Knockout cell line (C4-2B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MRPL40 Knockout cell line (C4-2B)
MRPL40 Knockout cell line (C4-2B)
Catalog#
YKO-H1106
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

Related Popular Products

Gene Knockout kit

EZ-cryo™ cell freezing medium

Related Popular Products

Gene Knockout kit

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

Contact us