MRPL40 Knockout cell line (C4-2B)

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MRPL40 Knockout cell line (C4-2B) Order

MRPL40 Knockout cell line (C4-2B)
Catalog#
YKO-H1106
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MRPL40 Knockout cell line (C4-2B)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MRPL40 Knockout cell line (C4-2B)
Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
MRPL40
Gene ID
64976
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Target region
Knockout region: Exon 1 (based on transcript MRPL40-201)
Validation: PCR+Sanger Sequencing
MRPL40 Knockout cell line (C4-2B) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MRPL40 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the C4-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MRPL40 gene knockout MRPL40 Knockout cell line (C4-2B) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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