Esr1 Knockout cell line(NIT-1)

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Esr1 Knockout cell line(NIT-1) Order

Esr1 Knockout cell line(NIT-1)
Catalog#
YKO-M063
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Esr1 Knockout cell line(NIT-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Esr1 Knockout cell line (NIT-1)
Morphology
Epithelial-like,adherent
Passage ratio
1:3~1:4
Species
Mouse
Gene Name
Esr1
Gene ID
13982
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM/F12+10% FBS
Target region
Knockout region: Exon 3 (based on transcript Esr1-204)
Validation: PCR+Sanger Sequencing
Esr1 Knockout cell line(NIT-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Esr1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NIT-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Esr1 gene knockout Esr1 Knockout cell line (NIT-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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