Sirt5 Knockout cell line(HL-1)

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Sirt5 Knockout cell line(HL-1) Order

Sirt5 Knockout cell line(HL-1)
Catalog#
YKO-M018
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Sirt5 Knockout cell line(HL-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Sirt5 Knockout cell line (HL-1)
Morphology
Fibroblast-like, adherent
Passage ratio
1:2-1:4
Species
Mouse
Gene Name
Sirt5
Gene ID
68346
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript Sirt5-207)
Validation: PCR+Sanger Sequencing
Sirt5 Knockout cell line(HL-1) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HL-1
STR Info (Cell bank)
Cell Line:HL-1
Allele1Allele2Allele3Allele4Allele1Allele2Allele3
4-2 234.09
【17.3】
233.52
【17.3】
5-5 340.08
【15】
339.64
【15】
6-4 300.26
【18】
299.95
【18】
6-7 334.91
【12】
334.38
【12】
9-2 221.61
【15】
221.02
【15】
12-1 226.26
【16】
225.65
【16】
15-3 213.4
【25.3】
212.89
【25.3】
18-3 152.75
【16】
152.82
【16】
X-1 400.48
【25】
404.48
【26】
400.86
【25】
404.88
【26】
D4S2408
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Sirt5 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HL-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Sirt5 gene knockout Sirt5 Knockout cell line (HL-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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