NFE2L2 Knockout cell line (U251MG)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > NFE2L2 Knockout cell line (U251MG)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

NFE2L2 Knockout cell line (U251MG) Order

NFE2L2 Knockout cell line (U251MG)
Catalog#
YKO-H924
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
NFE2L2 Knockout cell line (U251MG)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
NFE2L2 Knockout cell line (U251MG)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
NFE2L2
Gene ID
4780
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
Target region
Knockout region: Exon 2 (based on transcript NFE2L2-201)
Validation: PCR+Sanger Sequencing
NFE2L2 Knockout cell line (U251MG) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:U251MG
STR Info (Cell bank)
Cell Line:U251-MG
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 10111011
D7S820 10121012
D16S539 12121212
VWA 16181618
TH01 9.39.39.39.3
AMEL XXXX
TPOX 8888
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NFE2L2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the U251MG cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NFE2L2 gene knockout NFE2L2 Knockout cell line (U251MG) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

  • U251MG cell line

    Human Astrocytoma(U251MG)

    Catalog#: YC-D015
    Size: 1*10^6
    Instruction: Human Astrocytoma(U251MG)

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • NFE2L2 Knockout cell line (Hela)

    NFE2L2 Knockout cell line (Hela)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91081
    YKO-XN91081(Hela)
  • NFE2L2 Knockout cell line (HCT 116)

    NFE2L2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91080
    YKO-XN91080(HCT 116)
  • NFE2L2 Knockout cell line (HEK293)

    NFE2L2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91079
    YKO-XN91079(HEK293)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • NFE2L2 Knockout cell line (Hela)

    NFE2L2 Knockout cell line (Hela)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91081
  • NFE2L2 Knockout cell line (HCT 116)

    NFE2L2 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91080
  • NFE2L2 Knockout cell line (HEK293)

    NFE2L2 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: NFE2L2
    Gene ID: 4780
    Size: 1*10^6
    Catalog#: YKO-XN91079

Contact us