PIKFYVE Knockout cell line (HAP1)

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location: Home > Products > KO Cell Lines > PIKFYVE Knockout cell line (HAP1)
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PIKFYVE Knockout cell line (HAP1) Order

PIKFYVE Knockout cell line (HAP1)
Catalog#
YKO-H1177
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
PIKFYVE Knockout cell line (HAP1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
PIKFYVE Knockout cell line(HAP1)
Cell Morphology
 Adherent
Passage ratio
1:5-1:8
Species
Human
Gene Name
PIKFYVE
Gene ID
200576
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%IMDM+10%FBS
Target region
Knockout region: Exon 7 (based on transcript PIKFYVE-201)
Validation: PCR+Sanger Sequencing
PIKFYVE Knockout cell line (HAP1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIKFYVE gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HAP1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIKFYVE gene knockout PIKFYVE Knockout cell line(HAP1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
PIKFYVE Knockout cell line (HAP1)
PIKFYVE Knockout cell line (HAP1)
Catalog#
YKO-H1177
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Gene Name: PIKFYVE
    Gene ID: 200576
    Size: 1*10^6
    Catalog#: YKO-HS7775
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