Ahr Knockout cell line(H-4-II-E)

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Ahr Knockout cell line(H-4-II-E) Order

Ahr Knockout cell line(H-4-II-E)
Catalog#
YKO-RJ010
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Ahr Knockout cell line(H-4-II-E)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
Ahr Knockout cell line (H-4-II-E)
Species
Rat
Gene Name
Ahr
Gene ID
25690
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Ahr gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the H-4-II-E cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Ahr gene knockout Ahr Knockout cell line (H-4-II-E) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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