SHFL Knockout cell line (HeLa)

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SHFL Knockout cell line (HeLa) Order

SHFL Knockout cell line (HeLa)
Catalog#
YKO-H716
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SHFL Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SHFL Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
SHFL
Gene ID
55337
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 5 (based on transcript SHFL-201)
Validation: PCR+Sanger Sequencing
SHFL Knockout cell line (HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SHFL gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SHFL gene knockout SHFL Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SHFL Knockout cell line (HeLa)
SHFL Knockout cell line (HeLa)
Catalog#
YKO-H716
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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