SLC35C1 Knockout cell line (HepG2)

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SLC35C1 Knockout cell line (HepG2) Order

SLC35C1 Knockout cell line  (HepG2)
Catalog#
YKO-H871
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SLC35C1 Knockout cell line  (HepG2)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SLC35C1 Knockout cell line (HepG2)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SLC35C1
Gene ID
55343
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10% FBS
Target region
Knockout region: Exon 1 (based on transcript SLC35C1-201)
Validation: PCR+Sanger Sequencing
SLC35C1 Knockout cell line  (HepG2) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:Hep G2
STR Info (Cell bank)
Cell Line:GS-HepG2
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 913913
D7S820 10101010
D16S539 12121212
VWA 17171717
TH01 9999
AMEL XYXY
TPOX 8989
CSF1PO 10111011
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SLC35C1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SLC35C1 gene knockout SLC35C1 Knockout cell line (HepG2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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