SQSTM1 Knockout cell line (HT-29)

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SQSTM1 Knockout cell line (HT-29) Order

SQSTM1 Knockout cell line (HT-29)
Catalog#
YKO-H917
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SQSTM1 Knockout cell line (HT-29)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SQSTM1 Knockout cell line (HT-29)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
SQSTM1
Gene ID
8878
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 3 (based on transcript SQSTM1-202)
Validation: PCR+Sanger Sequencing
SQSTM1 Knockout cell line (HT-29) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HT-29
STR Info (Cell bank)
Cell Line:HT-29
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11121112
D7S820 10101010
D16S539 11121112
VWA 17191719
TH01 6969
AMEL XXXX
TPOX 8989
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SQSTM1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HT-29 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SQSTM1 gene knockout SQSTM1 Knockout cell line (HT-29) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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