TLN1 Knockout cell line(NB4)

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TLN1 Knockout cell line(NB4) Order

TLN1 Knockout cell line(NB4)
Catalog#
YKO-H400
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TLN1 Knockout cell line(NB4)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TLN1 Knockout cell line (NB4)
Morphology
Monocyte, suspension
Passage ratio
Control the cell density at 2x10^5~4.0x10^5 cells/ml during passaging, and carry out the passage when the cells grow to 8x10^5~1.0x10^6 cells/ml.
Species
Human
Gene Name
TLN1
Gene ID
7094
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Target region
Knockout region: Exon 6 (based on transcript TLN1-201)
Validation: PCR+Sanger Sequencing
TLN1 Knockout cell line(NB4) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:NB4
STR Info (Cell bank)
Cell Line:NB-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 101314.01013
D16S539 9999
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 811811
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NB4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLN1 gene knockout TLN1 Knockout cell line (NB4) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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