TMEM127 Knockout cell line (HeLa)

×

Subscribe Us

By subscription, you consent to allow Ubigene Biosciences to store and process the information provided above to deliver the latest news, research spotlight, and promotions. You can unsubscribe from these communications at anytime.
location: Home > Products > KO Cell Lines > TMEM127 Knockout cell line (HeLa)
All products
  • All products
  • Red Cotton™ gRNA Plasmid Bank
  • KO Cell Lines
  • Wild-type Cell Lines
  • Cas9 Stable Cell Lines
  • Luciferase Stable Cell Lines
  • EGFP Stable Cell Lines
  • Stem Cell Lines
  • Transfection Culture Medium
  • Cell Monoclonal Culture Medium
  • EZ-Stem™ Cell Culture Medium
  • EZ-cryo™ cell freezing medium
  • Kits
  • Lentivirus

TMEM127 Knockout cell line (HeLa) Order

TMEM127 Knockout cell line (HeLa)
Catalog#
YKO-H640
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM127 Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TMEM127 Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
TMEM127
Gene ID
55654
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript TMEM127-201)
Validation: PCR+Sanger Sequencing
TMEM127 Knockout cell line (HeLa) Target region
Loci STR Info (Sample Cell)
Sample Cell Line:HeLa
STR Info (Cell bank)
Cell Line:HELA
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 1213.31213.3
D7S820 812812
D16S539 910910
VWA 16181618
TH01 7777
AMEL XXXX
TPOX 812812
CSF1PO 910910
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM127 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM127 gene knockout TMEM127 Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

KO Cell Lines

  • TMEM127 Knockout cell line (HCT 116)

    TMEM127 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94767
    YKO-XN94767(HCT 116)
  • TMEM127 Knockout cell line (HEK293)

    TMEM127 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94766
    YKO-XN94766(HEK293)
  • TMEM127 Knockout cell line (A549)

    TMEM127 Knockout cell line (A549)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94765
    YKO-XN94765(A549)

Related Popular Products

Wild-type Cell Lines

Mouse Genotyping Kit

  • High-performance Mouse Genotyping Kit

    High-performance Mouse Genotyping Kit

    Price (USD): -;-;-
    Catalog#: YK-MGH-50;YK-MGH-100;YK-MGH-250
    Size: ~50 tests;~250 tests;~250 tests
    Instruction:

EZ-cryo™ cell freezing medium

  • EZ-cryo™ Cell Freezing Medium

    EZ-cryo™ Cell Freezing Medium

    Price (USD): -;-
    Catalog#: YK-CR-50;YK-CR-100
    Size: 50ml;100ml
    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TMEM127 Knockout cell line (HCT 116)

    TMEM127 Knockout cell line (HCT 116)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94767
  • TMEM127 Knockout cell line (HEK293)

    TMEM127 Knockout cell line (HEK293)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94766
  • TMEM127 Knockout cell line (A549)

    TMEM127 Knockout cell line (A549)

    Organism: Human
    Gene Name: TMEM127
    Gene ID: 55654
    Size: 1*10^6
    Catalog#: YKO-XN94765

Contact us