TMEM63B Knockout cell line (HeLa)

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TMEM63B Knockout cell line (HeLa) Order

TMEM63B Knockout cell line (HeLa)
Catalog#
YKO-H642
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM63B Knockout cell line (HeLa)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TMEM63B Knockout cell line (HeLa)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
TMEM63B
Gene ID
55362
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 2 (based on transcript TMEM63B-202)
Validation: PCR+Sanger Sequencing
TMEM63B Knockout cell line (HeLa) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM63B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HeLa cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM63B gene knockout TMEM63B Knockout cell line (HeLa) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TMEM63B Knockout cell line (HeLa)
TMEM63B Knockout cell line (HeLa)
Catalog#
YKO-H642
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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