TRAF4 Knockout cell line (HCT116)

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TRAF4 Knockout cell line (HCT116) Order

TRAF4 Knockout cell line (HCT116)
Catalog#
YKO-H1075
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TRAF4 Knockout cell line (HCT116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TRAF4 Knockout cell line (HCT116)
Species
Human
Gene Name
TRAF4
Gene ID
9618
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript TRAF4-201)
Validation: PCR+Sanger Sequencing
TRAF4 Knockout cell line (HCT116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TRAF4 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TRAF4 gene knockout TRAF4 Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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