UBE3A Knockout cell line (HEK293)

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UBE3A Knockout cell line (HEK293) Order

UBE3A Knockout cell line (HEK293)
Catalog#
YKO-H923
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
UBE3A Knockout cell line (HEK293)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
UBE3A Knockout cell line (HEK293)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
UBE3A
Gene ID
7337
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10% FBS
Target region
Knockout region: Exon 5 (based on transcript UBE3A-226)
Validation: PCR+Sanger Sequencing
UBE3A Knockout cell line (HEK293) Target region
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HEK293
STR Info (Cell bank)
Cell Line:HEK-293.2sus
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8888
D13S317 12141214
D7S820 11121112
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 12121212
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the UBE3A gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HEK293 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, UBE3A gene knockout UBE3A Knockout cell line (HEK293) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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