USP7 Knockout cell line (HCT 116)

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location： Home > Products > KO Cell Lines > USP7 Knockout cell line (HCT 116)

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USP7 Knockout cell line (HCT 116) Order

USP7 Knockout cell line (HCT 116)
Catalog#
YKO-HS6715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
USP7 Knockout cell line (HCT 116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
USP7 Knockout cell line (HCT 116)
Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
USP7
Gene ID
7874
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%McCOY's 5A+10% FBS
Target region
Knockout region: Exon 2 (based on transcript USP7-201)
Validation: PCR+Sanger Sequencing
USP7 Knockout cell line (HCT 116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the USP7 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, USP7 gene knockout USP7 Knockout cell line (HCT 116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
USP7 Knockout cell line (HCT 116)
USP7 Knockout cell line (HCT 116)
Catalog#
YKO-HS6715
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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