lncRNA-ITGA2 Knockout cell line(293T)

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lncRNA-ITGA2 Knockout cell line(293T) Order

lncRNA-ITGA2 Knockout cell line(293T)
Catalog#
YKO-H333
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
lncRNA-ITGA2 Knockout cell line(293T)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
lncRNA-ITGA2 Knockout cell line (293T)
Morphology
Epithelial-like, adherent
Passage ratio
1:3~1:6
Species
Human
Gene Name
lncRNA-ITGA2
Gene ID
——
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90% DMEM+10%FBS
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:293T
STR Info (Cell bank)
Cell Line:HEK293T
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 8989
D13S317 12121212
D7S820 11111111
D16S539 913913
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 11111111
CSF1PO 11121112
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the lncRNA-ITGA2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 293T cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, lncRNA-ITGA2 gene knockout lncRNA-ITGA2 Knockout cell line (293T) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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