HDAC7 Knockout cell line (HT-29)

CRISPR knockout cell lines are engineered through targeted genome editing to eliminate gene function, supporting studies of cellular biology, disease mechanisms, and drug discovery.

HDAC7 (histone deacetylase 7) encodes a member of the histone deacetylase family that regulates chromatin structure and gene expression through histone deacetylation. By modifying histone acetylation states, HDAC7 can influence accessibility of transcription factors to DNA and contribute to transcriptional repression. Its activity is therefore involved in regulation of gene expression, cell-cycle processes, and developmental programs.

HT-29 is a human colorectal adenocarcinoma cell line established from a primary colon tumor of a 44-year-old female patient in 1964. The cells can undergo differentiation into mature goblet-like cells and produce mucin-secreting subclones, such as HT29-MTX, which exhibit characteristics of intestinal epithelial differentiation, including tight junction formation and mucus layer production. HT-29 is widely used as a model for studying colorectal cancer biology, intestinal epithelial differentiation, barrier function, and drug or nanoparticle transport.

Each knockout cell line is validated by STR authentication, sterility testing (bacteria/fungi), and mycoplasma screening. Genotype is confirmed by two rounds of PCR and Sanger sequencing. Only clones that pass all QC criteria are released, ensuring reliable identity and performance for downstream applications.