HCT 116-Luc cell line

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  • HCT 116-Luc HCT 116-Luc-HCT 116-Luc
  • HCT 116-Luc cell line HCT 116-Luc-产品图
HCT 116-Luc Copy Product Info
Catalog#: YC-C004-Luc-P
Size: 1*10^6
Culture method: 90%McCOY's 5A+10% FBS
  • HCT 116-Luc HCT 116-Luc-HCT 116-Luc
  • HCT 116-Luc cell line HCT 116-Luc-产品图
HCT 116-Luc Copy Product Info
Catalog#: YC-C004-Luc-P
Size: 1*10^6
Culture method: 90%McCOY's 5A+10% FBS

Detailed Product Information

Cell Info

Product category Luciferase Stable Cell Lines Mycoplasma test negative
Cell Morphology Epithelial-like, adherent Culture method 90%McCOY's 5A+10% FBS
Passage ratio 1:2~1:4 Species Human
Reporter gene Luciferase Resistance gene puro
Accession
CVCL_C8WT(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Product category
Luciferase Stable Cell Lines
Mycoplasma test
negative
Cell Morphology
Epithelial-like, adherent
Culture method
90%McCOY's 5A+10% FBS
Passage ratio
1:2~1:4
Species
Human
Reporter gene
Luciferase
Resistance gene
puro
Accession
CVCL_C8WT(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.

Description

Luciferase is the general name of a class of enzymes that catalyze the oxidation and luminescence of luciferin or fatty aldehydes in organisms. Currently, firefly luciferase is the most commonly used enzyme in scientific research. This enzyme can catalyze the oxidative decarboxylation of D2 luciferin, produce activated oxidized luciferin, and emit reddish biological fluorescence. Because luciferase is based on the mutual reaction between enzyme and substrate, it has the characteristics of strong specificity, high sensitivity and signal-to-noise ratio, as well as strong penetration ability of biological fluorescent tissue. Ubigene's HCT 116-Luc cell line was constructed on the basis of wild-type HCT 116 cells by lentiviral transduction, which can stably express firefly luciferase for a long time. This cell line has low passage, high activity and good condition, and can be directly used for in vivo cell injection, which can well help you with the research of transcription factor regulation mechanism, in vivo imaging, cell tracking and other experiments.
luc产品描述
Imaging process of stable tumor cell lines in vivo
Luciferase validation
Sample Compound
pore 1
Compound
pore 2
Compound
pore 3
Average Expressed
multiple
HCT 116-Luc 325782 298167 343729 322559.333 261.535
HCT 116 997 1531 1172 1233.333
STR Profile
Loci STR Info (Sample Cell)
Sample Cell Line:HCT 116
STR Info (Cell bank)
Cell Line:HCT 116
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 10111011
D13S317 10121012
D7S820 11121112
D16S539 11131113
VWA 17221717
TH01 8989
AMEL XXXY
TPOX 8889
CSF1PO 710710
D12S391 1721
FGA 1823
D2S1338 1616
D21S11 2930
D18S51 1617
D8S1179 1214
D3S1358 1219
D6S1043 1313
PENTAE 1314
D19S433 1213
PENTAD 913
D1S1656 1214
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

  • icon
    Easy to use
    Luciferase Stable Cell Line in our cell bank can stably express luciferase. It can be directly used for in vivo injection to detect the tumorigenicity of cells.
  • icon
    Sensitive accurate
    Luciferase Stable Cell Line in our cell bank have high specificity and high sensitivity, enables high imaging quality, and its luminescence intensity can be accurately quantified.
  • icon
    Curated cells
    Our Luciferase Stable Cell Lines have been optimized and have low passages, high activity and good cell condition, applicable for detection or tumorigenesis experiment.

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