ARPE-19-Luc
Red cotton OmniCell bank
Online inquireOverview
Gene reporter systems are widely used in studies of eukaryotic gene expression and cellular physiology, serving as a common approach to improving experimental accuracy and reliability. The luciferase reporter system, which detects luciferase activity using luciferin or firefly aldehyde as substrates, is one of the most extensively applied reporter systems. Owing to its operational simplicity, high sensitivity, and high success rate, it is widely applied in gene expression research.
The ARPE-19-Luciferase cell line developed by Ubigene was generated using the lentivirus method. In addition to enabling stable and high-efficiency expression of the luciferase gene, it offers high specificity, excellent imaging quality, and accurately quantifiable luminescence intensity. This cell line supports a broad range of downstream applications, including promoter activity analysis, mammalian two-hybrid assays, and in vivo animal imaging studies. Its flexibility and quantitative performance make it reliable for gene regulation research, functional validation, and preclinical model development.
Detailed Product Information
ARPE-19 is a spontaneously arising retinal pigment epithelia (RPE) cell line derived in 1986 by Amy Aotaki-Keen from the normal eyes of a 19-year-old male who died from head trauma in a motor vehicle accident.The line was established in a 1:1 mixture of Dulbecco's modified Eagles medium and Ham's F12 medium with HEPES buffer containing 20% fetal bovine serum, 56 mM final concentration sodium bicarbonate and 2 mM L-glutamine and incubated at 37C in 10% CO2. The cells were subjected to selective trypsinization for the first four passages to remove superficial cells before passaging the cuboidal basal layer. By passage 5, the cultures appeared to be rapidly growing RPE cells, which would form cobblestone monolayers, which pigmented after several months in culture.
Product Advantages
Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
Plasmid Backbone Map
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Quality Control
| Sample Name | Duplicated well 1 | Duplicated well 2 | Duplicated well 3 | Average value | Ratio |
|---|---|---|---|---|---|
| ARPE-19-Luc | 902536 | 919771 | 949827 | 924044.667 | 256.086 |
| ARPE-19 | 3531 | 3622 | 3672 | 3608.333 |
Intended use
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use
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