3D4/21 cell line

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location: Home > Products > Wild-type Cell Lines > Porcine Alveolar Macrophage Cell Line(3D4/21)
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  • 3D4/21 cell line Porcine Alveolar Macrophage Cell Line(3D4/21)-产品图
Porcine Alveolar Macrophage Cell Line(3D4/21) Copy Product Info
Catalog#: YC-C041
Size: 1*10^6
Culture method: 85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose
  • 3D4/21 cell line Porcine Alveolar Macrophage Cell Line(3D4/21)-产品图
Porcine Alveolar Macrophage Cell Line(3D4/21) Copy Product Info
Catalog#: YC-C041
Size: 1*10^6
Culture method: 85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose

Detailed Product Information

Cell Info

Product category Wild-type Cell Lines Mycoplasma test negative
Cell Morphology Macrophage-like, adherent Culture method 85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose
Passage ratio 1:2~1:3 Species Pig
Accession
CVCL_0F14(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Product category
Wild-type Cell Lines
Cell Morphology
Macrophage-like, adherent
Passage ratio
1:2~1:3
Mycoplasma test
negative
Culture method
85%RPMI-1640+10%FBS+1%Glutamax+1%SodiumPyruvate+1%NEAA+1%Hepes+2.2g/ml Glucose
Species
Pig
Accession
CVCL_0F14(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
The parental porcine monomyeloid cell line, 3D4, was established in December of 1998 following transfection of primary porcine alveolar macrophage cultures with the pSV3neo plasmid. Single cell cloning and selection in G-418 of the 3D4 parental cell line resulted in establishment of 3D4/2 (ATCC CRL-2845), 3D4/21 (ATCC CRL-2843) and 3D4/31 (ATCC CRL-2844).

Recommended Transfection Method

Electroporation
Lentivirus Transduction

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