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DNAJA3 Stable Expression Cell Line (SH-SY5Y)

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DNAJA3 Stable Expression Cell Line (SH-SY5Y)
Size
1*10^6
Culture method
90%DMEM+10% FBS
Instruction
STR Report
Detailed Product Information
DNAJA3 Stable Expression Cell Line (SH-SY5Y)

This gene encodes a member of the DNAJ/Hsp40 protein family. DNAJ/Hsp40 proteins stimulate the ATPase activity of Hsp70 chaperones and play critical roles in protein folding, degradation, and multimeric complex assembly. The encoded protein is localized to mitochondria and mediates several cellular processes including proliferation, survival and apoptotic signal transduction. The encoded protein also plays a critical role in tumor suppression through interactions with oncogenic proteins including ErbB2 and the p53 tumor suppressor protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Product category
Stable Cell Lines
Transcript
NM_005147.6
Cell Name
DNAJA3 Stable Expression Cell Line (SH-SY5Y)
Species
Human
Gene Name
DNAJA3
Passage ratio
1:2-1:3
Cell Morphology
Epithelial-like, adherent
Antibiotic Concentration
Puro=2μg/ml
Culture method
90%DMEM+10% FBS
Mycoplasma test
negative
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
Gene Expression Validation
Sample NameDNAJA3-1CтMeanβ-h_actin CтMeanΔCтMeanΔΔCтExpression Rate
DNAJA3 Stable Expression Cell Line (SH-SY5Y)19.22513.3565.869-2.4735.552
SH-SY5Y_Ctrl(Puro)22.95814.6168.3420.0001.000

Intended use

For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use

Unpacking and Storage Instructions

1.
Cell Reception
2.
Unpacking and storage instructions

Handling procedure

1.
Cell Thawing
2.
Cell Passaging
3.
Cell cryopreservation

Product Advantage

Easy to use
Easy to use

Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations

High-efficient KO
High-efficient KO

These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.

Curated cells
Curated cells

Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.

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