Direct Capture of Guide RNAs Enables Scalable and Combinatorial Single-Cell CRISPR Screens


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Direct Capture of Guide RNAs Enables Scalable and Combinatorial Single-Cell CRISPR Screens

Single-cell CRISPR screens enable the exploration of mammalian gene function and genetic regulatory networks. Recently, multiple techniques have emerged that pair CRISPR screens with high-throughput single-cell RNA sequencing (scRNA-seq), resulting in high resolution, information-rich readouts.

In this webinar brought to you by The Scientist and sponsored by 10x Genomics, Dina Finan will introduce the 10x Genomics product portfolio, including new targeted gene expression panels. Joining Dina will be Joseph Replogle who will discuss how this technology helped him and colleagues develop direct capture Perturb-seq, a new single cell CRISPR screening technique that greatly expands accessibility, scalability, and flexibility of single cell CRISPR experiments.

Topics to be covered

Streamlining pooled single-cell CRISPR screens with combinatorial perturbation libraries
Improving efficacy of CRISPR interference and activation by targeting individual genes with multiple sgRNAs
Applying direct capture Perturb-seq to study the genetic interactions (GIs) between cholesterol biosynthesis and DNA repair genes

Leveraging hybridization-based target enrichment gene panels to decrease sequencing cost up to 90%

Ubigene Biosciences is co-founded by biological academics and elites from China, the United States, and France. We are located in Guangzhou Science City, which serves as a global center for high technology and innovation. Ubigene Biosciences has 1000㎡ office areas and laboratories, involving genome editing, cell biology technology, and zebrafish research. We provide products and services for plasmids, viruses, cells, and zebrafish. We aim to provide customers with better gene-editing tools for cell or animal research.

We developed CRISPR-U™ and CRISPR-B™(based on CRISPR/Cas9 technology) which is more efficient than general CRISPR/Cas9 in double-strand breaking, CRISPR-U™ and CRISPR-B™ can greatly improve the efficiency of homologous recombination, easily achieve knockout (KO), point mutation (PM) and knockin (KI) in vitro and in vivo. 

Genome Editing Platform
——Focusing on the Application of CRISPR-U™ and CRISPR-B™ Gene Editing Technology
1. Provides various types of gene-editing vectors for different species.
2. Provides different virus packaging services, including lentiviruses, adenoviruses and adeno-associated viruses.3. Provides high-quality services for gene knockout, point mutation and knockin cell lines

Cell Biology Platform
——Focusing on primary cell
1. Provides over 400 types of primary cells.
2. Provides culture strategies and related products for different cell types.3. Provides cell biology-related services such as cell isolation, extraction and validation.

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