Since the release of Red CottonTM CRISPR gene-editing design system (haven’t used yet? Click for more details), its powerful and smart functions for gene-editing strategy design has reaped a wave of good comments with its advantages of rapidity, precision, intelligence, etc. Meanwhile, some users have expressed the hope that microbial gene editing can also have a place in Red Cotton system. Well, only you can't think of it, nothing we can't do. Microbial gene-editing strategy design system is now released!
Are you still struggling with:
No idea of how to find the target gene information?
Are microbial knockouts better with traditional homologous recombination methos or CRISPR / cas9 technology?
How do I design gRNAs without understanding microbial gene-editing?
No idea of how to design the most appropriate knockout region?
Combining the gene-editing big data, bioinformatics and information technology, Ubigene added an updated function of [Microbial gene-editing strategy design] to the existing functions in Red Cotton system, covering various microbial species of Escherichia coli, Salmonella, Bacillus subtilis, Shigella castellani, Saccharomyces cerevisiae, etc. In 1 min, you can get the automatically-generated gene-editing strategy for free, one-stop addressing your scientific research troubles!
Simply visit this link https://www.rc-crispr.com and it will jump to the Red Cotton system.
Click [Microbe], and it will switch the search page to the microbial panel.
Choose [Species] and [Strain], enter [Gene name or ID or Locus tag], click the search bottom to proceed with the searching.
Count to three with the cartoon, and you will get the results!
Select the strategy information of your target by clicking [View], wait for 1 min, and the amazing KO strategy will come out!