Ubigene officially establishes our
EZ-editor™ Gene-editing Series Products
Good Experimental materials are always required for the easier experiment >
With 12 years of experience, Ubigene has exclusively innovated and developed 6 product lines
Help you achieve "Easier genome editing"
Now Ubigene also releases the Gene-editing Cell Value Pack
Applicable for all types of wild-type cells to achieve gene-editing
Paired with various of gene-editing useful tools
One-stop addressing all your needs - From cell culture to genotype validation for single-cell clones
20% OFF for the Luxury Value Pack
Save your time, save your effort, help you easily achieve gene-editing
Monoclone Validation Kit (extraction free)
This Monoclone Validation Kit (extraction free) is exclusively developed by Ubigene's expert team, it includes genomic sample preparation and PCR system. And it minimizes cell sample usage with a streamlined genomic sample preparation process, which effectively resolves some issues such as the time-consuming gene-editing experiment, complicated steps for single-cell clone validation, and large costs of the experiment. The kit has a wide range of applications, with a large body of experimental data demonstrating its suitability for all types of adherent versus suspension cells, including but not limited to cells of human, mouse, rat or other animal origin, and supports all types of gene editing experiments for positive clone validation.
A large amount of DNA template amounts from cell samples (as low as 100 cells ) can be obtained for PCR experiments in only 3 steps using this kit, which achieves the purpose of high-throughput and rapid identification of genotype in early growth stage of clones, screening qualified single clones in advance, and eliminating the cost of culturing large numbers of non-positive clones. The kit contains preferred PCR identification reagents and can be used to compatible with multiple impurities remaining in genomic crude extract samples and other factors that may affect PCR reactions, improving the accuracy of identification results . In addition, two tools developed by Ubigene, (1) the primer design tool for gene editing target site identification (high efficiency primer design platform) provided in the Red Cotton™ system and (2) EZ-editor™ Genotype Analysis System (GAS) can assist you more rapidly through the entire single-cell clone validation process.
Wild-type Cell Lines, Stable Cell Lines (Cas9, Luciferase)
Based on the high requirement of in-stock cells, Ubigene has established two exclusive cell banks: Wild-type Cell Bank, Tool Cell Bank ( Cas9 Stable Cell Line and Luciferase Stable Cell Line ). The wild-type cell lines in our cell bank are selected by Ubigene from more than a thousand cases of gene editing experiments , which are the most preferred and suitable WT cells for gene-editing experiments. Our in-stock tool cell lines include high-quality Cas9 cell lines and Luc cell lines , bringing great convenience for researchers and these cells can shorten the experimental turnaround. The cells in the two cell banks have low passages, high activity, and all cells of human and murine origin in our cell bank have available STR Authentication reports, guaranteeing accurate cell identity. Ubigene will provide the professional technical support of gene-editing technology for free.
Transfection Culture Medium
For the characteristics of different experimental phases and different cells in the gene-editing experiment, two types of specific Culture Medium are developed by Ubigene, Transfection Culture Medium and Cell Monoclonal Culture Medium . Transfection Culture Medium helps optimize cell condition before transfection, ensure the transfection effect, and effectively improve the gene editing efficiency. Cell Monoclonal Culture Medium can improve the monoclonal formation rate of cells . For cells that are inherently difficult to form single-cell clones, Ubigene has successfully optimized the medium proportion and formula to improve the rate. Experimental data show that the use of these 2 Culture Mediums can effectively improve gene-editing efficiency.
To enjoy gene-editing, Gene-editing Cell Line Package is enough
Each package contains the following products:
High-quality Wild-type cell line
Transfection Culture Medium
Cell Monoclonal Culture Medium
Monoclone Validation Kit
Monoclone Validation Kit
Promotion period: Valid until 10/31/2021
Over 10,000 in-stock gRNA plasmids as low as $80
Based on our CRISPR-U™ Technology and thousands of gene-editing success cases , Ubigene has successfully developed a series of gRNA plasmids (YKO) with high gene-editing efficiency, including regular plasmids, lentiviral plasmids, and AAV plasmids. And Ubigene has successfully established the Red Cotton™ gRNA Plasmid Bank, which is the basis of our "Red Cotton Gene Knockout Project". Ubigene's plasmids can enter cells via regular transfection methods and can edit target sites efficiently after transient expression of high levels of Cas9 protein in target cells. Also, our plasmids can be co-transfected with a variety of gRNAs to target multiple sites in cells. At present, our Red Cotton™ gRNA Plasmid Bank has over 10000 gRNA plasmids in stock, which can be widely used in different fields of gene-editing research. You can search our in-stock gRNA plasmids according to your gene of interest.
Cas9/GFP/mCherry/Luciferase expression Lentivirus
Lentivirus, different from regular retrovirus, can effectively infect cells in division and non-division, and the infection efficiency is very high, which is several times or even dozens of times that of traditional methods. Lentivirus has a wide range for host and can effectively infect many types of cells, such as neurons, hepatocytes, cardiomyocytes, tumor cells, endothelial cells, stem cells, etc. In addition, it also has the advantages of low immunogenicity and large gene capacity. It is a powerful tool for introducing foreign genes, and Lentivirus is often used to construct stable cell lines . While ensuring the Strong activity, high titer and stable expression of fluorescence/resistance genes of lentivirus vector, Ubigene adopts the third generation lentivirus packaging system for lentivirus packaging to make it safer.
Mycoplasma Treatment Kit
The Mycoplasma Treatment kit can specifically address Mycoplasma contamination, launched by Ubigene expert team based on our own extensive cell culture experience. The experimental data shows that it takes as fast as 3 days to effectively remove mycoplasma inside and outside the cells as quickly as possible using our Mycoplasma Treatment kit, which also eliminates Common Gram negative or positive bacteria to some extent. Thus it can resolve the problems due to mycoplasma contamination and ensure the accuracy of cell research results.