Detection of Marker Protein Expression

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Detection of Marker Protein Expression
Immunofluorescence technique, also known as Fluorescent Antibody Technique, is one of the earliest development of labeled immune technology. It is a technology based on immunology, biochemistry and microscopy. For a long time, some scholars have tried to combine antibody molecules with some tracer substances, and use antigen antibody reaction to locate antigen substances in tissues or cells.

Technical details
Immunofluorescence technique is based on the principle of antigen-antibody reaction. Firstly, the known antigen or antibody is labeled with fluorescent groups, and then the fluorescent antibody (or antigen) is used as a probe to detect the corresponding antigen (or antibody) in cells or tissues. The nature and location of the antibody can be seen by fluorescence microscope.



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Reference
Im, Kyuseok et al. “An Introduction to Performing Immunofluorescence Staining.” Methods in molecular biology (Clifton, N.J.) vol. 1897 (2019): 299-311. doi:10.1007/978-1-4939-8935-5_26


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