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  • How does CRISPR/Cas9 work for RKO cell line research?

    Background RKO cell line is a low differentiated colon cancer cell line developed by Michael Brattain. It contains wild-type P53, but it lacks human thyroid receptor nuclear receptor (h-TRβ1). RKO cell line is the parental cell line of RKO-E6 and RKO-AS45-1, and its P53 protein l…

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