Engineered THP-1 cell line: models for immune diseases and inflammatory research
A human leukemic cell line (THP-1) cultured from the blood of a boy with acute monocytic leukemia. It plays an important role in the studies of regulating tissue homeostasis and inflammatory response. THP-1 cells have been widely used in the research of monocyte and macrophage related mechanisms, signaling pathways, nutrient and drug transportation, and immune response. Compare with U937, HL-60 and ML-2, the morphological and functional characteristics of THP-1 are very similar to human primary monocytes (including cell differentiation markers). Compared with human peripheral blood monocytes (PBMC), THP-1 is easier to be cultured and has a more consistent background. Therefore, THP-1 is a commonly used acute monocytic leukemia cell line in various laboratories, and it is an ideal tool for studying immunity and inflammation.
CRISPR/Cas9 mediated KO THP-1 cell line, a advanced model for the study of GD
Gaucher disease (GD) is an autosomal recessive lysosomal storage disorder caused by mutations in the acid β-glucosidase gene (GBA1), resulting in the deficient activity of acid β-glucosidase (GCase), and the subsequent progressive accumulation of glucosylceramide (GlcCer) and glucosylsphingosine (LysoGL1) within the lysosomes. Researchers have focused on developing effective GD cell models that can reproduce the genetic and phenotypic characteristics of the disease, in order to seek better pathophysiological research and develop new treatments. Eleonora Pavan et al. exploited CRISPR/Cas9 technology to edit GBA1 in the human monocytic THP-1 cell line to develop an isogenic GD model of monocytes.
Schematic representation of GBA1 editing workflow is shown in Fig.1A. WB analysis of THP-1 GBA1 mutant D2, D6 and F5 clones showed lower levels of GCase expression in comparison to THP-1 wt (Fig. 1B). And GCase enzymatic activity was significantly reduced in all three clones (Fig.1C). Among them, D2 clone had almost no enzyme activity (the remaining activity of wild-type was 1%). Because GlcCer accumulated in GD cells is transformed by lysosomal acid ceramidase into its deacetylated lipase LysoGL1, it is considered to be a biomarker of GD. Therefore, they detected LysoGL1 in all mutant clones and found that a massive LysoGL1 accumulation was detected in THP-1 GBA1 mutant clone D2 (Fig.1D). Thus, the researchers chose D2 as the GD model. THP-1 GBA1 mutant cells provide a good model for further exploring the pathophysiology of GD, and can be used as an alternative research object for some cells that are difficult to obtain and difficult to proliferate in vitro, such as monocytes from GD patients, macrophages differentiated from monocytes or macrophages differentiated from IPSC.
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Nrf2-KO THP-1 cell line facilitate the study of SLE treatment
Systemic lupus erythematosus (SLE) is a multifactorial autoimmune disease. The transcription factor nuclear-factor-E2-related factor 2(Nrf2, also known asNFE2L2) mediates the transcription and expression of many antioxidant and anti-inflammatory genes. Nrf2 deficiency leads to the development of lupus-like autoimmune nephritis in aged female mice, indicating that Nrf2 is vital to the development and progression of SLE. A recent study by Mills et al. demonstrated that itaconate is a novel activator of Nrf2. Itaconate directly alkylates cysteine residues 151, 257, 288, 273, and 297 on Keap1, causing Nrf2 protein stabilization, accumulation, and activation. Additionally, a new cell-permeable itaconate derivative, 4-octyl itaconate (OI), increases the expression of Nrf2 downstream genes with antioxidant and anti-inflammatory capacities. In order to confirm that OI induced anti-inflammatory effect requires the activation of Nrf2, the researchers used shRNA to inhibit Nrf2 and constructed Nrf2 KO cell line to completely knockout Nrf2 in THP-1 cell line. As shown, the expression level of Nrf2 mRNA was significantly reduced in stable THP-1 cells with Nrf2 shRNA or the Nrf2-KO construct (Fig.2A). OI-induced Nrf2 protein stabilization (Fig. 2A), HO1/NQO1mRNA (Fig. 2C), and protein (Fig. 2B) expression, as well as NQO1 activation (Fig. 2D), were almost blocked by Nrf2 silencing or KO (Fig. 4B-D). Importantly, OI pre-treatment did not inhibit LPS-induced NFκB activation (Fig. 2E) and TNF-α production (Fig. 2F) in Nrf2-silenced or -KO THP-1 cells.
It proves that Nrf2 activation was required for OI-induced anti-inflammatory actions in THP-1 human macrophages and SLE patient-derived PBMCs. Nrf2 silencing or knockout (by CRISPR/Cas9 gene-editing method) almost abolished OI-induced anti-oxidant and anti-inflammatory actions in SLE patient-derived PBMCs and LPS-activated THP-1 cells. A key advantage of this new compound over other Nrf2 activators is that it is directly related to Keap1 and leads to Keap1 acetylation and Nrf2 dissociation, resulting in Nrf2 direct and continuous activation. Thus, OI and itaconate have important therapeutic value for the treatment of SLE.
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The overexpression of IL-38 in THP-1 causes anti-inflammatory effect
Interleukin (IL)-38 is a cytokine belonging to the IL-1 family. This cytokine is expressed in the synovium of rheumatoid arthritis (RA). It has been found that mice lacking IL-38 exacerbate arthritis. In order to study whether the overexpression of IL-38 in THP-1 monocyte line in vitro affects its cytokine production, the researchers used lentivirus transfection of THP-1 cells to overexpress human IL-38 (Fig. 3A, B). The results showed that overexpression of IL-38 in THP-1 significantly reduced the expression of IL-6 and IL-23p19 mRNA stimulated by lipopolysaccharide (LPS). In addition, it also significantly reduced LPS induced IL-6 and TNF α, IL-23 and IL-10 proteins secrete 2.5 times (Fig. 3C, D), but IL-1 β was not affected (Fig. 3D).
This study emphasizes the anti-inflammatory effect of IL-38 overexpression in vivo: this cytokine acts on macrophages in inflammatory infiltration, has an indirect effect on Th17 cytokines, and reduces the expression of pro-inflammatory cytokines. However, whether IL-38 can be considered as a new treatment option for arthritis or other inflammatory diseases needs further experimental research.
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